A scoping review and evidence map of radiofrequency field exposure and genotoxicity: assessing in vivo, in vitro, and epidemiological data
Implications for policy and practice
The evidence from the evidence map indicates that medium to long-term RF-EMF exposures, particularly at low intensities, can induce genetic damage through non-thermal mechanisms such as increased free radical production and oxidative stress. Genetic damage can have far-reaching, long-term, and potentially irreversible consequences for individual organisms and broader ecological and planetary health (112, 113).
Both In vivo and epidemiological RF-EMF studies provide credible evidence of genotoxicity, suggesting potential risks such as increased cancer susceptibility and reproductive harm. Studies on brain cells frequently reported positive findings for DNA damage, suggesting that brain cells may be particularly sensitive to RF-EMF, indicating a risk for neurological diseases and brain tumors, as observed in animal models (114–116).
Current RF-EMF exposure guidelines established by ICNIRP (15), prioritize the prevention of thermal effects by incorporating substantial safety margins (e.g., a 50-fold reduction from effect thresholds, setting a local SAR limit of 2 W/kg for the head and torso for the general public, averaged over 10 grams of tissue). However, the evidence mapping process found statistically significant DNA damage at extremely low intensities, with the lowest recorded effects occurring at a SAR of 0.000000319 W/kg in an epidemiological study (117) and at 0.000003 W/kg in several in vivo experiments (118, 119). These levels are substantially (>600,000 times) below the ICNIRP public exposure limits (15). This pattern suggests non-thermal genotoxic effects, because temperature changes at these intensities would be negligible and not measurable.
ICNIRP (2020) guidelines (15) set RF-EMF exposure limits to protect against thermal effects from acute exposures, with averaging times of 6 min for local exposure (head and torso) and 30 min for whole-body exposure. However, the above analysis revealed that medium (1 day−3 months) and long-term RF-EMF exposures (>3 months or 1,000 h) were most strongly linked to genotoxic effects, even at very low exposure intensities. ICNIRP (2020) guidelines (15) do not set specific limits for chronic, low-level RF-EMF exposures, particularly for non-thermal effects like genotoxicity, citing “no substantiated evidence of health-relevant effects” [(15), p. 522].
The mapping process also revealed that RF exposures are associated with genetic damage in a wide range of organisms, with an observed sensitivity of non-mammalian organisms, such as plants, insects, and possibly amphibians. Current guidelines neglect potential effects on wildlife or ecosystems (78, 96). Notably, a recent WHO-commissioned systematic review of animal studies suggested carcinogenic effects from RF-EMF exposures (116). Other studies suggest biological effects on non-human species (120, 121). Together, these results suggest that the environmental implications of RF-EMF exposure merit greater scrutiny (122), even though the current evidence remains limited and debated (96).
While these findings do not yet establish causation or a clear No Observed Adverse Effect Level (NOAEL), they indicate risks that ICNIRP's current framework discounts by prioritizing only effects with confirmed harm [(15), p. 487]. ICNIRP's review process and position is best described as a hazard-based assessment focused only on confirmed effects. This approach is overly restrictive, as it delays updating guidelines until absolute certainty is achieved (123), which may not align with the precautionary needs of public health or environmental protection.
Currently, there is a widespread (6) and often non-consensual nature to RF-EMF exposure (92) from mobile phones, base stations, and other wireless technologies. While acknowledging the permanence of this technology in modern society, policy adjustments are required that prioritize health and environmental protection over economic interests. This can be achieved by adopting a precautionary approach to RF-EMF (123) and addressing potential risks from non-thermal RF-EMF effects, despite scientific uncertainty. Strategies such as justification (assessing net benefits of RF-EMF applications), optimization (balancing protection with societal needs), and As low as Reasonably Achievable or As Low as Technically Achievable - ALARA/ALATA (avoiding deterministic effects and minimizing stochastic effects) per International Commission on Radiological Protection (ICRP) recommendations - ICRP103 (124) could be considered. Further development and deployment of wireless technologies should incorporate improved safety measures in their design (125), such as creating devices that emit lower levels of RF-EMF or using materials and antenna designs to direct emissions away from the body.
Additionally, public information regarding potential health risks and personal protective measures could be disseminated through public health campaigns, making use of existing advice such as the EUROPAEM EMF Guideline 2016 (126); e.g. minimizing the use of wireless devices, prioritizing wired connections, maintaining distance between RF-EMF sources and the body, use of air-tube headsets or handsfree calls, turning off wireless when not in use, and mitigation of oxidative stress by incorporating antioxidants into the diet.
While individual actions are valuable, they are not a substitute for robust regulatory standards and industry accountability. Ensuring the safety of wireless technologies requires a collective effort from manufacturers, policymakers, and consumers to develop comprehensive RF safety guidelines. Future regulatory guidelines could encompass workplace protection measures, including substitution, engineering, and administrative controls (127), integration of building biology standards (128), mandatory detailed product labeling to inform users of potential risks, and standardized safety hygiene practices.
To address these concerns and bridge existing gaps, the following actions are recommended:
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Standardization of Research Protocols: Harmonizing methodologies across studies, particularly comet assay protocols, is critical for reducing heterogeneity and enabling robust meta-analyses.
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Focus on Long-Term and Low-Intensity Exposures: Future research should prioritize investigating the cumulative effects of prolonged and low-intensity RF-EMF exposures, which are most relevant to real-world scenarios and devices.
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Inclusion of Emerging Frequencies: Given the rapid deployment of 5G and other novel technologies, research focused on higher frequencies and new modulation schemes is urgently needed.
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Targeted Environmental and Health Studies: Targeted research in both human health and ecological systems needs to be conducted independently of vested interest influences, ensuring methodological rigor in each domain.
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Independent Funding and Research Oversight: To mitigate biases associated with industry funding, greater support for independent research is essential. Transparent disclosure of ALL funding sources and researcher affiliations should be mandatory.
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Re-evaluation of RF Standards: Regulatory bodies must update exposure guidelines to reflect non-thermal mechanisms and the potential health effects from long-term chronic exposure settings by incorporating findings from independent, high-quality studies.
The evidence map presented here reveals statistically significant DNA damage in humans and animals resulting from man-made RF-EMF exposures, particularly DNA base damage and DNA strand breaks. The evidence also suggests plausible mechanistic pathways for DNA damage, most notably through increased free radical production and oxidative stress. Sensitivity to damage varied by cell type, with reproductive cells (testicular, sperm and ovarian) along with brain cells appearing particularly vulnerable. A complex U-shaped dose-response relationship was observed for both exposure duration and intensity, with more DNA damage occurring in specific frequency and intensity combination windows. DNA damage was more likely to be found using in vivo studies, very weak or very strong signal intensities, very short or very long exposure durations, 900, 1,800 and 2,450 MHz frequencies, GSM-talk mode and pulsed modulations, particularly when using real-world devices. On the other hand, research funded by vested interests has tended to use different experimental design parameters, with a high proportion of studies using in vitro, short-term exposures, medium-high intensity signals and using signal generators. Funding source is also a stronger determinant of experimental outcomes than study quality.
Overall, there is a strong evidence base showing DNA damage and potential biological mechanisms operating at intensity levels much lower than the ICNIRP recommended exposure limits. Public policy could benefit from the implementation of precautionary measures such as ALARA or ALATA, along with public information campaigns to better safeguard human and environmental health and wellbeing.
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Genetic effects of non-ionizing electromagnetic fields
Lai H. Genetic effects of non-ionizing electromagnetic fields. Electromagn Biol Med. 2021 Apr 3;40(2):264-273. doi: 10.1080/15368378.2021.1881866.
This is a review of the research on the genetic effects of non-ionizing electromagnetic field (EMF), mainly on radiofrequency radiation (RFR) and static and extremely low frequency EMF (ELF-EMF). The majority of the studies are on genotoxicity (e.g., DNA damage, chromatin conformation changes, etc.) and gene expression. Genetic effects of EMF depend on various factors, including field parameters and characteristics (frequency, intensity, wave-shape), cell type, and exposure duration. The types of gene expression affected (e.g., genes involved in cell cycle arrest, apoptosis and stress responses, heat-shock proteins) are consistent with the findings that EMF causes genetic damages. Many studies reported effects in cells and animals after exposure to EMF at intensities similar to those in the public and occupational environments. The mechanisms by which effects are induced by EMF are basically unknown. Involvement of free radicals is a likely possibility. EMF also interacts synergistically with different entities on genetic functions. Interactions, particularly with chemotherapeutic compounds, raise the possibility of using EMF as an adjuvant for cancer treatment to increase the efficacy and decrease side effects of traditional chemotherapeutic drugs. Other data, such as adaptive effects and mitotic spindle aberrations after EMF exposure, further support the notion that EMF causes genetic effects in living organisms.
"Supplements 1 and 2 show that the majority of studies reported genetic effects of EMF (66% for RFR and 79% for static/ELF-EMF). Thus, it is safe to conclude that genotoxic effects of EMF have been reported. The most common effects found are: DNA strand breaks, micronucleus formation, and chromosomal structural changes. There are not many studies on mutation. Thus, it is not known whether these genotoxic effects transform into mutation and involved in carcinogenesis. Interestingly, available data do not suggest mutagenic effect after RFR exposure (Chang et al., 2005; Meltz et al., 1990; Ono et al., 2004; Takahashi et al., 2002); whereas most static/ELF-EMF studies (Chahal et al., 1993; Mairs et al., 2007; Miyakoshi, 1997; Miyakoshi et al., 1998, 1996; Potenza et al., 2004; Wilson et al., 2015) suggested some mutagenic effects...."
"Effects of EMF on cellular free radical processes have been reported in many experiments (cf. Lai, 2019; Yakymenko et al., 2016). It is conceivable that an increase in free radicals in cells could cause macromolecular damages including DNA. There are many reports on involvements of free radicals in genetic processes, including both reactive oxygen species and reactive nitrogen species...."
To prove an effect, one should look for consistency in data. Genetic damage studies have shown similar effects with different set-up and in various biological systems. And, the gene expression results (Supplement 3) also support the studies on genetic damages. Expression of genes related to cell differentiation and growth, apoptosis, free radical activity, DNA repair, and heat-shock proteins have been reported. These changes could be consequences of EMF-induced genetic damages.... In conclusion, there are enough reasons to believe that genetic effects of EMF are real and possible.
During cell phone use, a relatively constant mass of tissue in the brain is exposed to the radiation at relatively high intensity (peak specific absorption rate (SAR) of 4–8 W/kg). Many papers have reported genetic effect/DNA damage at much lower SAR (or power density) (see Supplement 4). This questions the wisdom of the several exposure standard-setting organizations in using the obsolete data of 4 W/kg (whole-body averaged SAR) as the threshold for exposure-standard setting. Furthermore, since critical genetic mutations in one single cell are sufficient to lead to cancer and there are millions of cells in a gram of tissue, it is inconceivable that some standards have changed the SAR from averaged over 1 gm to 10 gm of tissue. (The limit of localized tissue exposure has been changed from 1.6 W/kg averaged over 1 gm of tissue to 2 W/kg over 10 gm of tissue. Since distribution of radiofrequency energy is non-homogenous inside tissues, this change allows a higher peak level of exposure.) What is actually needed is a better refinement of SAR calculation to identify ‘peak values’ of SAR inside the brain.
Any effect of EMF has to depend on the energy absorbed by a biological entity and on how the energy is delivered in space and time. Aside from influences that are not directly related to experimentation (Huss et al., 2007), many factors could influence the outcome of an experiment in bioelectromagnetics research. Frequency, intensity, exposure duration, and the number of exposure episodes can affect the response, and these factors can interact with each other to produce different effects. In addition, in order to understand the biological consequences of EMF exposure, one must know whether the effect is cumulative, whether compensatory responses result, and when homeostasis will break down. A drawback in the interpretation and understanding of experimental data from bioelectromagnetic research is that there is no general accepted mechanism on how EMF affects biological systems. Since the energy level is not sufficient to cause direct breakage of chemical bonds within molecules, the effects are probably indirect and secondary to other induced chemical changes in the cell. The mechanisms by which EMF causes genetic effects are unknown. This author suspects that biological effects of EMF exposure are caused by multiple inter-dependent biological mechanisms."